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Ammon
science forum beginner
Joined: 27 Jun 2006
Posts: 8
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 Posted: Tue Jun 27, 2006 6:39 pm Post subject:
Zinc Chloride solubility in water?
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I am trying to do a reverse stain on a SDS-PAGE by first using .2M
Imidazole and then .3M Zinc Chloride. I can't get the ZnCl2 to go into
solution. I have tried heating and even bought a new bottle of ZnCl2.
Any suggestion or help would be greatly appreciated. Thanks. |
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Ron Jones
science forum Guru Wannabe
Joined: 16 May 2005
Posts: 175
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| Posted: Tue Jun 27, 2006 7:22 pm Post subject:
Re: Zinc Chloride solubility in water?
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Ammon wrote:
| Quote: | I am trying to do a reverse stain on a SDS-PAGE by first using .2M
Imidazole and then .3M Zinc Chloride. I can't get the ZnCl2 to go
into solution. I have tried heating and even bought a new bottle of
ZnCl2. Any suggestion or help would be greatly appreciated. Thanks.
|
It's very soluble, just leaving it out of the bottle and it will make it's
own solution.
432g dissolves in 100ml of water. Are you sure you are using water? Is the
water basic? - try adding a drop of HCl.
--
Ron Jones
Process Safety & Development Specialist
Don't repeat history, unreported chemical lab/plant near missesa at
http://www.crhf.org.uk Only two things are certain: The universe and
human stupidity; and I'm not certain about the universe. ~ Albert
Einstein |
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number6
science forum addict
Joined: 29 Apr 2005
Posts: 67
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| Posted: Wed Jun 28, 2006 12:58 am Post subject:
Re: Zinc Chloride solubility in water?
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Ammon wrote:
| Quote: | I am trying to do a reverse stain on a SDS-PAGE by first using .2M
Imidazole and then .3M Zinc Chloride. I can't get the ZnCl2 to go into
solution. I have tried heating and even bought a new bottle of ZnCl2.
Any suggestion or help would be greatly appreciated. Thanks.
|
Zinc Chloride is very soluble in water ... we used to sell it in levels
as high as 70 weight per cent ... You may be getting some hydrolysis
.... or the Zinc Oxide that is generally present to a certain amount
shows up as cloudiness when one tries to dissolve it ...
Add a little HCl to clear it up ...
You can buy 50 % Zinc Chloride USP grade in various places ... |
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Ammon
science forum beginner
Joined: 27 Jun 2006
Posts: 8
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| Posted: Thu Jun 29, 2006 7:03 pm Post subject:
Re: Zinc Chloride solubility in water?
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Ron Jones wrote:
| Quote: | Ammon wrote:
I am trying to do a reverse stain on a SDS-PAGE by first using .2M
Imidazole and then .3M Zinc Chloride. I can't get the ZnCl2 to go
into solution. I have tried heating and even bought a new bottle of
ZnCl2. Any suggestion or help would be greatly appreciated. Thanks.
It's very soluble, just leaving it out of the bottle and it will make it's
own solution.
432g dissolves in 100ml of water. Are you sure you are using water? Is the
water basic? - try adding a drop of HCl.
--
Ron Jones
Process Safety & Development Specialist
Don't repeat history, unreported chemical lab/plant near missesa at
http://www.crhf.org.uk Only two things are certain: The universe and
human stupidity; and I'm not certain about the universe. ~ Albert
Einstein
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Thanks for the imput. Yes I am using water. ddH2O. ph=7.68. I added
240 microliters of 1M HCl. I ZnCl2 still didn't go into solution and
it did not proberly stain the gel. I am still at a loss. Everything I
have read says that it should be soluble, but it is acting different
than expected. |
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rekuci@gmail.com
science forum addict
Joined: 22 Sep 2005
Posts: 98
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| Posted: Thu Jun 29, 2006 7:43 pm Post subject:
Re: Zinc Chloride solubility in water?
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Ammon wrote:
| Quote: | I am trying to do a reverse stain on a SDS-PAGE by first using .2M
Imidazole and then .3M Zinc Chloride. I can't get the ZnCl2 to go into
solution. I have tried heating and even bought a new bottle of ZnCl2.
Any suggestion or help would be greatly appreciated. Thanks.
|
If it's not dissolving in water, and HCl does nothing, then the only
explanation is that what you have is NOT zinc chloride. Get a 'new
bottle' from somewhere else than where you've been getting it, you're
wasting time and there's not much help anyone can give you but the
facts (i.e., it's VERY soluble). |
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