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Na contents measure using Atomic absorption spectrometry
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Bob M
science forum addict


Joined: 15 May 2005
Posts: 53

PostPosted: Sun May 15, 2005 5:53 pm    Post subject: Re: Tubing with hydrophilic coating inside the tube Reply with quote

Plastics are traditionally made hydrophilic by oxidising the surface
with acid dichromate or acid permanganate.
Treated polyethylene can be glued to other materials after the
procedure.

BobM
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MICHAEL RINALDI
science forum beginner


Joined: 15 Jun 2005
Posts: 3

PostPosted: Wed Jun 15, 2005 9:12 pm    Post subject: Re: Merck/Hitachi HPLC Manager file format Reply with quote

Hi: Try this. It reads almost everything. RAW is one of the file types it
lists.

http://www.xnview.com/

Mike

"Friedrich Menges" <effemm2@gmx.de> wrote in message
news:42554b24$1@merkur.rz.uni-konstanz.de...
Quote:
Hi all,

I have a couple of HPLC binary data files from an old Merck HPLC system,
controlled by a DOS software: D-6000 HPLC Manager Version 2. The only
output besides ugly needle printer printouts are binary files with the
extension *.rw1 and *.rw2
I would be very thankful for any hints about a software that is capable
of reading those files. I have to be able to read those files!

Thanks,
Friedrich
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MICHAEL RINALDI
science forum beginner


Joined: 15 Jun 2005
Posts: 3

PostPosted: Wed Jun 15, 2005 9:27 pm    Post subject: Re: Question about Hitachi´s F-4500 Flourescence Spectrometer Reply with quote

Hi: First of all the F-4500 is not an "old" instrument & is still actively
sold. Bio-luminescence is done with the lamp turned off. Set your
Excitation Monochromator to zero order, add your reagents and scan your
Emission Monochromater (speed depends upon the rate of your reaction) for
bands of interest. Set your Emission Mono on the strongest peak, mix
another batch and now scan your Excitation Mono for strongest band. You now
have the EX & EM bands needed to do your work.
Mike
"Christian Weber" <chri.weber@tu-bs.de> wrote in message
news:d2r70d$b52$1@rzcomm2.rz.tu-bs.de...
Quote:
Hello!

We´re trying to implement two luciferase-based luminescence assays on our
"aged" F-4500 which schould be capable of measuring luminescence according
to the manufacturer´s manual. Because the manual only contains few
information about luminescence-reading and because the manufacturer
doesn´t
supply technical support on this older machine any longer we are facing
some
problems...

Did anyone of you make some experiences with bio-luminescence measured by
the F-4500? We would be glad to receive any hints how to improve our
measuring procedure.

Tanks a lot

Christian Weber

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Dominic-Luc Webb
science forum beginner


Joined: 16 Jun 2005
Posts: 1

PostPosted: Thu Jun 16, 2005 5:09 am    Post subject: Re: Question about Hitachi´s F-4500 Flouresc ence Spectrometer Reply with quote

On Wed, 15 Jun 2005, MICHAEL RINALDI wrote:

Quote:
Hi: First of all the F-4500 is not an "old" instrument & is still actively
sold.


True, but it seems this individual has an old machine. Mine is also
quite old. One issue regarding the use of this machine for luciferase
is that the enzymes are not free and at the same time, the cuvettes
are the older, classical 3 ml type. Many people doing this assay
use 96 well fluorescent plate readers, which is about 0.3 ml. This
tenfold reduction in volume makes a significant economic difference.
Further, it is often convenient to run a large number of samples with
a plate rather than one reading from one cuvette at a time.

I have been looking for smaller volume fused silica cuvettes. If anyone
knows about these and any adapters for the F4500, that would be great
for me, and maybe for the original poster as well.

I can't contribute anything further regarding usin this machine for
bioluminescence, but it is clear it can be done, taking into consideration
the proviso mentioned above.



Dominic-Luc Webb



Bio-luminescence is done with the lamp turned off. Set your
Quote:
Excitation Monochromator to zero order, add your reagents and scan your
Emission Monochromater (speed depends upon the rate of your reaction) for
bands of interest. Set your Emission Mono on the strongest peak, mix
another batch and now scan your Excitation Mono for strongest band. You now
have the EX & EM bands needed to do your work.
Mike
"Christian Weber" <chri.weber@tu-bs.de> wrote in message
news:d2r70d$b52$1@rzcomm2.rz.tu-bs.de...
Hello!

We´re trying to implement two luciferase-based luminescence assays on our
"aged" F-4500 which schould be capable of measuring luminescence according
to the manufacturer´s manual. Because the manual only contains few
information about luminescence-reading and because the manufacturer
doesn´t
supply technical support on this older machine any longer we are facing
some
problems...

Did anyone of you make some experiences with bio-luminescence measured by
the F-4500? We would be glad to receive any hints how to improve our
measuring procedure.

Tanks a lot

Christian Weber




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George Orwell
science forum beginner


Joined: 04 May 2005
Posts: 49

PostPosted: Thu Jun 16, 2005 12:54 pm    Post subject: Re: Merck/Hitachi HPLC Manager file format Reply with quote

"MICHAEL RINALDI" <vze2s95r@verizon.net> wrote in
news:wB2se.4185$aR1.3527@trndny02:

You surely can't be serious. Suggesting that a multimedia viewer
might be able to read (and make sense of) some manufacturer's
proprietary chromatographic data files is ludicrous.

George

Quote:
Hi: Try this. It reads almost everything. RAW is one of the
file types it lists.

http://www.xnview.com/

Mike

"Friedrich Menges" <effemm2@gmx.de> wrote in message
news:42554b24$1@merkur.rz.uni-konstanz.de...
Hi all,

I have a couple of HPLC binary data files from an old Merck
HPLC system, controlled by a DOS software: D-6000 HPLC Manager
Version 2. The only output besides ugly needle printer
printouts are binary files with the extension *.rw1 and *.rw2
I would be very thankful for any hints about a software that is
capable of reading those files. I have to be able to read those
files!

Thanks,
Friedrich

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MICHAEL RINALDI
science forum beginner


Joined: 15 Jun 2005
Posts: 3

PostPosted: Thu Jun 16, 2005 1:48 pm    Post subject: Re: Merck/Hitachi HPLC Manager file format Reply with quote

George: Sorry, I tried to help. Please re-read your original post
requesting any hints, I'm not that stupid. If as you say they are
"proprietary" then other than the original S/W puts you SOL.

"George Orwell" <the_pig@farm.com.invalid> wrote in message
news:Xns9677506C495EAorwellian@216.196.97.131...
Quote:
"MICHAEL RINALDI" <vze2s95r@verizon.net> wrote in
news:wB2se.4185$aR1.3527@trndny02:

You surely can't be serious. Suggesting that a multimedia viewer
might be able to read (and make sense of) some manufacturer's
proprietary chromatographic data files is ludicrous.

George

Hi: Try this. It reads almost everything. RAW is one of the
file types it lists.

http://www.xnview.com/

Mike

"Friedrich Menges" <effemm2@gmx.de> wrote in message
news:42554b24$1@merkur.rz.uni-konstanz.de...
Hi all,

I have a couple of HPLC binary data files from an old Merck
HPLC system, controlled by a DOS software: D-6000 HPLC Manager
Version 2. The only output besides ugly needle printer
printouts are binary files with the extension *.rw1 and *.rw2
I would be very thankful for any hints about a software that is
capable of reading those files. I have to be able to read those
files!

Thanks,
Friedrich


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Dave White
science forum beginner


Joined: 18 Jun 2005
Posts: 1

PostPosted: Sat Jun 18, 2005 12:18 am    Post subject: Re: Merck/Hitachi HPLC Manager file format Reply with quote

"MICHAEL RINALDI" <vze2s95r@verizon.net> wrote in message
news:7bhse.2626$EH1.1980@trndny03...
Quote:

"proprietary" then other than the original S/W puts you SOL.


Not totally true - I have the file formats for a number of mass spec and GC
systems. Unfortunately, I don't have this one.


--
Dave White
SpectraChrom Software
www.spectrachrom.com
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Jim1166
science forum beginner


Joined: 15 Jun 2005
Posts: 13

PostPosted: Sat Jul 02, 2005 4:52 pm    Post subject: Re: Shimadzu LOWLIFES Reply with quote

Koichi Tanaka wrote:

(Extraneous snipped)

Quote:
paid $312,000 for this home on August 11, 1998

assessed value of this home was $182,470 on 1/1/97

paid $125,000 for this home on November 30, 1984
assessed value of this home was $201,660 on 1/1/98

paid $399,990 for this home on August 13, 1997

paid $252,100 for this home on August 30, 1995


paid $280,000 for this home on April 4, 1993

paid $97,500 for this condo on June 26, 1987

assessed value of this home was $168,840 on 1/1/97

assessed value of this home was $170,570 (base) on 1/1/97


Looks like Shimadzu must be a pretty reputable company that makes great
competitive equipment.

I can't imagine all their higher ups being paid all this money, and
Shimadzu still being able to stay in business, if it wasn't for the
great reliable equipment they sell!!! Especially when some other major
companies are cutting back benefits.

Jim
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Friedrich Menges
science forum beginner


Joined: 06 Jul 2005
Posts: 2

PostPosted: Wed Jul 06, 2005 6:47 pm    Post subject: Re: Merck/Hitachi HPLC Manager file format Reply with quote

Hi there,

the problem is solved in the meantime. There is a small DOS conversion
software from MERCK, which I received from a friend who already had this
problem earlier.
It's me who originally posted the request, but I have to agree with
"George Orwell". The files are data files, not graphics files, so there
is not much use for a graphics program. To him this was a clear issue,
you didn't know...

Thanks to all,
Friedrich.

MICHAEL RINALDI wrote:

Quote:
George: Sorry, I tried to help. Please re-read your original post
requesting any hints, I'm not that stupid. If as you say they are
"proprietary" then other than the original S/W puts you SOL.

"George Orwell" <the_pig@farm.com.invalid> wrote in message
news:Xns9677506C495EAorwellian@216.196.97.131...

"MICHAEL RINALDI" <vze2s95r@verizon.net> wrote in
news:wB2se.4185$aR1.3527@trndny02:

You surely can't be serious. Suggesting that a multimedia viewer
might be able to read (and make sense of) some manufacturer's
proprietary chromatographic data files is ludicrous.

George


Hi: Try this. It reads almost everything. RAW is one of the
file types it lists.

http://www.xnview.com/

Mike

"Friedrich Menges" <effemm2@gmx.de> wrote in message
news:42554b24$1@merkur.rz.uni-konstanz.de...

Hi all,

I have a couple of HPLC binary data files from an old Merck
HPLC system, controlled by a DOS software: D-6000 HPLC Manager
Version 2. The only output besides ugly needle printer
printouts are binary files with the extension *.rw1 and *.rw2
I would be very thankful for any hints about a software that is
capable of reading those files. I have to be able to read those
files!

Thanks,
Friedrich



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Jim1166
science forum beginner


Joined: 15 Jun 2005
Posts: 13

PostPosted: Sat Jul 09, 2005 6:33 pm    Post subject: Re: Look familiar Joel Polowin? Reply with quote

MA Sonjariv wrote:

Quote:
If you BUY S***, then you'll HAVE S*** period!!!!

Sad--


Your mom should have washed your mouth out with soap.

No professional speaks like that. Gues George Orwell really has you
pegged!!!
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George Orwell
science forum beginner


Joined: 04 May 2005
Posts: 49

PostPosted: Sun Jul 10, 2005 1:41 am    Post subject: Re: Look familiar Joel Polowin? Reply with quote

Jim <nospam@anonymous.net> wrote in
news:8OidnZGVR4YVqU3fRVn-tQ@adelphia.com:

Quote:
MA Sonjariv wrote:

If you BUY S***, then you'll HAVE S*** period!!!!

Sad--

Your mom should have washed your mouth out with soap.

No professional speaks like that. Gues George Orwell really has
you pegged!!!


Not just pegged, but nailed to the wall. Apparently not content with
his Japanese personae, he now adopts the posting nym of his critic,
MA Sonjariv. Imitation is the best flattery, I suppose.

I wonder who Joel Polowin is, and what he did to get on Mark's wrong
side? Although it seems that simply being on the planet is grounds
for being on Mark's wrong side.

Of course, he thinks -I'm- Joel Polowin, just like a few months ago
when he though I was someone else. Poor sad Markie. Doesn't it just
rile you that you have no idea who your nemeses really are?

George
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Borek
science forum Guru Wannabe


Joined: 02 May 2005
Posts: 157

PostPosted: Sat Jul 16, 2005 8:49 pm    Post subject: Re: Production of inorganic alcohol ? Reply with quote

On Sat, 16 Jul 2005 18:21:00 +0200, Marvin <physchem@cloud9.net> wrote:

Quote:
I assumed he meant a liquid fluid in general.

Pardon the ignorance of a non-chemist, but is there a thing
like "non liquid fluid"?

Best,
Borek
--
Check pH lectures at www.chembuddy.com - comments welcomed!

http://www.chembuddy.com - chemical calculators for labs and education
BATE - program for pH calculations
CASC - Concentration and Solution Calculator
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Guest






PostPosted: Wed Aug 03, 2005 2:19 pm    Post subject: Re: Searching for Shimadzu in the Scientific Literature ?? Reply with quote

TO: Mr. MA Sonjariv (masonjar (at)hotmail.com) or
whoever you are

Why do you keep posting Kukucka's name and
position? According to your info, he's a National
Sales & Marketing Manager. Sounds like he's done
very well for himself! What title do you hold
other than Chief Instigator & Harasser?

What proof can you offer that Kukukca is behind
any of this? Answer the question Mr. Sonjariv! Or
are you afraid of the truth!

Lastly, the bashing of Shimadzu seems warranted.
Name another company that has the multitude of
problems as outlined at:

http://shimadzu.wxs.org
http://shimadzu.home-page.org
http://shimadzu-sux.iscool.net

List facts Mr. Sonjariv because we ALL know that
you'd rather attack the messenger because you
can't stand the message!

You're attempt to censor the message will never
succeed! The truth is out there for those who
want to know!
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MA Sonjariv
science forum beginner


Joined: 03 May 2005
Posts: 10

PostPosted: Thu Aug 04, 2005 4:08 am    Post subject: Re: Searching for Shimadzu in the Scientific Literature ?? Reply with quote

On Wed, 03 Aug 2005 16:19:29 GMT, contact abuse@shaw.ca wrote:

Quote:
TO: Mr. MA Sonjariv (masonjar (at)hotmail.com) or
whoever you are

Why do you keep posting Kukucka's name and
position? According to your info, he's a National
Sales & Marketing Manager. Sounds like he's done
very well for himself! What title do you hold
other than Chief Instigator & Harasser?

What proof can you offer that Kukukca is behind
any of this? Answer the question Mr. Sonjariv! Or
are you afraid of the truth!

Lastly, the bashing of Shimadzu seems warranted.
Name another company that has the multitude of
problems as outlined at:

http://shimadzu.wxs.org
http://shimadzu.home-page.org
http://shimadzu-sux.iscool.net

List facts Mr. Sonjariv because we ALL know that
you'd rather attack the messenger because you
can't stand the message!

You're attempt to censor the message will never
succeed! The truth is out there for those who
want to know!

http://www.geocities.com/kukuckapage/

Check the above link for the details..

I wonder what Kukucka's employer will say - or do - when they find out
they have a certified mental case in their company.

hmm ...

As we get closer to August 6th, expect the rants and the babbling to
increase from the kukucka!

Mikhail Alexi Sonjariv, PhD
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Guest






PostPosted: Thu Aug 04, 2005 11:30 am    Post subject: Re: Searching for Shimadzu in the Scientific Literature ?? Reply with quote

Quote:

Mikhail Alexi Sonjariv, PhD


That name looks like another pile of s**t-madzu!

Can't seem to find your name associated with any
scientific publications in "Current Contents" for
the last 2 decades or so... hmmmm!

Sounds like identity theft to me!!!

So what Russian nuclear missle program have you
been working on Mikhail... or is it Mikhaila?

And what piece(s) of Shimadzu equipment have you
used to publish your phantom publications with?

Somebody likened you to Joel Polowin a few weeks
ago... could it be Joel masquerading again???
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